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News

We also had 2 of our students presenting posters at the conference. Visit https://www.embl.org/about/info/course-and-conference-office/events/omx24-01/#vf-tabs__section-overview to learn more about the conference and other speakers that will be present

You can read more about the conference program at https://www.standardbio.com/cytofsummit2024#speakers-anchor

Read more of our archived news updates and stay up to date by following us on Twitter.


Research

Translational Research

As part of the Pancreatic Cancer Translational Research Initiative at the Ontario Institute of Cancer Research, we are studying how intra- and inter-tumour cellular heterogeneity contributes to therapeutic resistance and poor outcomes in Pancreatic Ductal AdenoCarcinoma. By utilizing serial sections on a large cohort to simultaneously quantify single cell phenotypes and markers of their functional state, as well as their interactions, overall organization, and contribution to tissue architecture, we are able to identify multi-cellular networks that can inform clinical decision making like never done before. This work has been a vital collaboration between our graduate students, Ferris, Sybil, Tiak, and post doctoral fellow, Noor.
https://oicr.on.ca/programs/pancreatic-cancer-translational-research-initiative-pancurx/

By pairing mouse models of mammary tumorigenesis and highly multiplexed cellular profiling of human breast cancers we are investigating the molecular pathways which drive breast cancer initiation and metastasis. For this purpose, we are working closely with scientific collaborators within the Lunenfeld Tanenbaum Researcher Institute and the clinical teams who have created the Breast Translational Research Resource at Mount Sinai Hospital. And as part of the Terry Fox Research Institute Hippo Pathway Team, we have developed a spatially resolved functional genomics screening approach to dissect the extracellular signaling networks controlled by tumour-specific Hippo signaling. This work is spear headed by our post doctoral fellow, Eddy.

Methods Development

We develop spatially resolved systems biology tools and analysis methods to quantify complex tissue systems across various biological scales; from genomes to pathway specific signaling, and even how these influence cellular phenotype and tissue level patterning. With specific expertise in Imaging Mass Cytometry and CyTOF technology, we combine spatially resolved ‘omics’ methods to investigate the molecular underpinnings of classic histopathology and unravel the complexity of tumour morphology and architecture. We are continually improving the quantitative methods we use, developing new reagents to measure cellular function, and creating new functional and phenotypic screening approaches. Join our lab to find out more and get involved with the ever-evolving field.

With thanks to all of our funding sources for their generous support of our work.


Publications

Recent Publications

Select Publications

  • Jackson, H.W., Fischer, J.R., Zanotelli, V.R.T. et al. The single-cell pathology landscape of breast cancer. Nature 578, 615–620 (2020). https://doi.org/10.1038/s41586-019-1876-x.
  • Ali, H.R., Jackson, H.W., Zanotelli, V.R.T. et al. Imaging mass cytometry and multiplatform genomics define the phenogenomic landscape of breast cancer. Nat Cancer 1, 163–175 (2020). https://doi.org/10.1038/s43018-020-0026-6
  • Schapiro, D., Jackson, H., Raghuraman, S. et al. histoCAT: analysis of cell phenotypes and interactions in multiplex image cytometry data. Nat Methods 14, 873–876 (2017). https://doi.org/10.1038/nmeth.4391

All Publications


People

Current Members

Hartland Jackson, PhD
Principle Investigator
hjackson@lunenfeld.ca

Anuroopa Dinesh
Lab Manager and
Research Technician
adinesh@lunenfeld.ca

Katie Teng
Graduate Student
kteng@lunenfeld.ca

Eddy Chen, PhD
Post Doctoral Fellow
echen@lunenfeld.ca

Noor Shakfa, PhD
Post Doctoral Fellow
nshakfa@lunenfeld.ca

Sibyl Drissler
Graduate Student
sdrissler@lunenfeld.ca

Jennifer Gorman, PhD
Research Manager and
Scientific Associate
gorman@lunenfeld.ca

Beth Sunnucks
Lab Manager and
Research Technician
sunnucks@lunenfeld.ca

Ching Yeung Lam
Graduate Student
chingyeung@lunenfeld.ca

Darren Chan
Graduate Student
dchan@lunenfeld.ca

Tiak Tan
Graduate Student
tiaktan@lunenfeld.ca

Isabelle Hébert-Milette, PhD
Post Doctoral Fellow
hebert-milette@lunenfeld.ca

Pearl Sequeira
Research Administration
sequeira@lunenfeld.ca

Matt Watson
Computational Scientist
mwatson@lunenfeld.ca

Ferris Nowlan
Graduate Student
fnowlan@lunenfeld.ca

Golnaz Abazari
Graduate Student
gabazari@lunenfeld.ca

Jennifer Pfeil
Graduate Student
jpfeil@lunenfeld.ca

Naila Abid
Graduate Student
nabid@lunenfeld.ca

Past Members

Simon Latour, PhD
Post Doctoral Fellow

Deni van Schie
Graduate Student

Ricardo Gonzalez, MD, PhD
Pathologist

Ari Forman
Graduate Student

Somi Afiuni, PhD
Computational Scientist

Collaborations

We are a highly collaborative team many partnerships within the institute as well as with other labs across Toronto and beyond.

  • We are developing new analysis methods for image cytometry and highly multiplexed imaging with the Campbell Lab at the Lunenfeld Tanenbaum Research Institute. https://www.camlab.ca/
  • With the neighbouring Schramek Lab at LTRI we have created mouse model systems for the functional genomics study of single cells and multi-cellular tissues. https://schramek.lunenfeld.ca/
  • Alongside the Notta Lab and the PanCuRx Initiative within the Ontario Institute for Cancer Research, we are investigating the single cell content of pancreatic ductal adenocarcinoma using a combination of ‘omics’ profiling methods. https://www.nottalab.com/
  • In collaboration with the McGuigan Lab in BioEngineering at the University of Toronto, we are engineering multi-cellular tissue environments at scale. http://www.mcguiganlab.com/
  • Working to develop new reagents for CyTOF and Imaging Mass Cytometry we work closely with the Nitz Lab in the Department of Chemistry at the University of Toronto. https://sites.chem.utoronto.ca/nitz/

Lab Life

How to Join

Even though our lab is currently close to capacity we are still always looking for creative and enthusiastic scientists to join our team. In particular, we are looking to recruit talented post-doctoral fellows who believe they would have a unique skill set to contribute to our lab. If you have a scientific research goals that you believe will align with ours, please contact Dr. Hartland Jackson directly to discuss your work and future interests.


Contact

We are located at:

Lunenfeld-Tanenbaum Research Institute
Mount Sinai Hospital
Joseph & Wolf Lebovic Health Complex

Room 881 - 600 University Avenue
Toronto, Ontario Canada M5G 1X5
(416) 586-4800 x 8267